Arginine-rich hydrophobic polyethylenimine: Potent agent with simple components for nucleic acid delivery

Hamideh Parhiz; Maryam Hashemi; Arash Hatefi; Wayne Thomas Shier; Sara Amel Farzad; Mohammad Ramezani

doi:10.1016/j.ijbiomac.2013.05.001

Arginine-rich hydrophobic polyethylenimine: Potent agent with simple components for nucleic acid delivery

Hamideh Parhiz, Maryam Hashemi, Arash Hatefi, Wayne Thomas Shier, Sara Amel Farzad, Mohammad Ramezani

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Conjugation of various arginine-rich peptide sequences to vectors based on 10. kDa polyethylenimine (PEI) and its hydrophobic derivative (hexanoate-PEI) was investigated as a strategy for improving pDNA and siRNA transfection activities. Six different arginine-histidine (RH) sequences and two arginine-serine (RS) sequences with a range of R/H ratios were designed and coupled to PEI and hexanoate-PEI. All arginine-rich peptide derivatives of PEI significantly enhanced luciferase gene expression compared to PEI 10. kDa alone. Hexanoate-PEI derivatives exhibited higher transfection activity than underivatized PEI vectors. Improved transfection activity may have resulted at least in part from use of higher vector/DNA ratios made possible by reduced cytotoxicity of vectors, and to use of vectors with higher molecular weights. Vectors that were the most efficient in pDNA delivery and transfection were also the most effective in siRNA delivery and protein expression knock down.

Original language	English (US)
Pages (from-to)	18-27
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	60
DOIs	https://doi.org/10.1016/j.ijbiomac.2013.05.001
State	Published - Sep 2013

Bibliographical note

Funding Information:
This work was supported by Mashhad University of Medical Sciences, Mashhad, Iran and the Iranian Nanotechnology Initiative . This study was part of H. Parhiz's PhD thesis. The siRNA experiments were performed at Pharmaceutical Biotechnology Center of Drug Research, Ludwig-Maximilians-University, Munich under supervision of Alex Philipp and Prof. Ernst Wagner.

Keywords

Arginine rich peptide
Gene therapy
Histidine rich peptide
Polyethylenimine
SiRNA

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10.1016/j.ijbiomac.2013.05.001

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Arginine-rich hydrophobic polyethylenimine : Potent agent with simple components for nucleic acid delivery. / Parhiz, Hamideh; Hashemi, Maryam; Hatefi, Arash; Shier, Wayne Thomas; Amel Farzad, Sara; Ramezani, Mohammad.

In: International Journal of Biological Macromolecules, Vol. 60, 09.2013, p. 18-27.

Research output: Contribution to journal › Article › peer-review

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abstract = "Conjugation of various arginine-rich peptide sequences to vectors based on 10. kDa polyethylenimine (PEI) and its hydrophobic derivative (hexanoate-PEI) was investigated as a strategy for improving pDNA and siRNA transfection activities. Six different arginine-histidine (RH) sequences and two arginine-serine (RS) sequences with a range of R/H ratios were designed and coupled to PEI and hexanoate-PEI. All arginine-rich peptide derivatives of PEI significantly enhanced luciferase gene expression compared to PEI 10. kDa alone. Hexanoate-PEI derivatives exhibited higher transfection activity than underivatized PEI vectors. Improved transfection activity may have resulted at least in part from use of higher vector/DNA ratios made possible by reduced cytotoxicity of vectors, and to use of vectors with higher molecular weights. Vectors that were the most efficient in pDNA delivery and transfection were also the most effective in siRNA delivery and protein expression knock down.",

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AU - Shier, Wayne Thomas

AU - Amel Farzad, Sara

AU - Ramezani, Mohammad

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N2 - Conjugation of various arginine-rich peptide sequences to vectors based on 10. kDa polyethylenimine (PEI) and its hydrophobic derivative (hexanoate-PEI) was investigated as a strategy for improving pDNA and siRNA transfection activities. Six different arginine-histidine (RH) sequences and two arginine-serine (RS) sequences with a range of R/H ratios were designed and coupled to PEI and hexanoate-PEI. All arginine-rich peptide derivatives of PEI significantly enhanced luciferase gene expression compared to PEI 10. kDa alone. Hexanoate-PEI derivatives exhibited higher transfection activity than underivatized PEI vectors. Improved transfection activity may have resulted at least in part from use of higher vector/DNA ratios made possible by reduced cytotoxicity of vectors, and to use of vectors with higher molecular weights. Vectors that were the most efficient in pDNA delivery and transfection were also the most effective in siRNA delivery and protein expression knock down.

AB - Conjugation of various arginine-rich peptide sequences to vectors based on 10. kDa polyethylenimine (PEI) and its hydrophobic derivative (hexanoate-PEI) was investigated as a strategy for improving pDNA and siRNA transfection activities. Six different arginine-histidine (RH) sequences and two arginine-serine (RS) sequences with a range of R/H ratios were designed and coupled to PEI and hexanoate-PEI. All arginine-rich peptide derivatives of PEI significantly enhanced luciferase gene expression compared to PEI 10. kDa alone. Hexanoate-PEI derivatives exhibited higher transfection activity than underivatized PEI vectors. Improved transfection activity may have resulted at least in part from use of higher vector/DNA ratios made possible by reduced cytotoxicity of vectors, and to use of vectors with higher molecular weights. Vectors that were the most efficient in pDNA delivery and transfection were also the most effective in siRNA delivery and protein expression knock down.

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Arginine-rich hydrophobic polyethylenimine: Potent agent with simple components for nucleic acid delivery

Abstract

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