ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats

Eun Mee Kim; Eugene O'Hare; Martha K. Grace; Catherine Welch; Charles J Billington; Allen S Levine

doi:10.1016/S0006-8993(00)02060-6

ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats

Eun Mee Kim
, Eugene O'Hare
, Martha K. Grace
, Catherine Welch
, Charles J Billington
, Allen S Levine

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and α-melanocyte stimulating hormone (α-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P>0.05). Obese Zucker rats had lower α-MSH and dynorphin A_1-17 peptide levels in the paraventricular nucleus (PVN) (P<0.05), but did not have lower PVN β-endorphin peptide levels (P>0.05). The decrease in POMC in the ARC and decrease in α-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. Copyright (C) 2000.

Original language	English (US)
Pages (from-to)	11-16
Number of pages	6
Journal	Brain Research
Volume	862
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)02060-6
State	Published - Apr 17 2000

Bibliographical note

Funding Information:
The authors are grateful to J.O. Douglass and S.L. Sabol for generously providing transformed cell stocks needed to produce the cDNA probes for POMC, proDYN, proENK, and proNPY. This research was supported by General Research Funds of the Department of Veterans Affairs, Minnesota Obesity Center Grant NIDDK DK-50456, and by NIDA Grant DA-03999.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Energy regulation
Melanocortin
NPY
Opioids

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10.1016/S0006-8993(00)02060-6

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@article{0a5dccb2eaa64ea991adb6afc972241b,

title = "ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats",

abstract = "Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and α-melanocyte stimulating hormone (α-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P>0.05). Obese Zucker rats had lower α-MSH and dynorphin A1-17 peptide levels in the paraventricular nucleus (PVN) (P<0.05), but did not have lower PVN β-endorphin peptide levels (P>0.05). The decrease in POMC in the ARC and decrease in α-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. Copyright (C) 2000.",

keywords = "Energy regulation, Melanocortin, NPY, Opioids",

author = "Kim, \{Eun Mee\} and Eugene O'Hare and Grace, \{Martha K.\} and Catherine Welch and Billington, \{Charles J\} and Levine, \{Allen S\}",

note = "Funding Information: The authors are grateful to J.O. Douglass and S.L. Sabol for generously providing transformed cell stocks needed to produce the cDNA probes for POMC, proDYN, proENK, and proNPY. This research was supported by General Research Funds of the Department of Veterans Affairs, Minnesota Obesity Center Grant NIDDK DK-50456, and by NIDA Grant DA-03999.",

year = "2000",

month = apr,

day = "17",

doi = "10.1016/S0006-8993(00)02060-6",

language = "English (US)",

volume = "862",

pages = "11--16",

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T1 - ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats

AU - Kim, Eun Mee

AU - O'Hare, Eugene

AU - Grace, Martha K.

AU - Welch, Catherine

AU - Billington, Charles J

AU - Levine, Allen S

N1 - Funding Information: The authors are grateful to J.O. Douglass and S.L. Sabol for generously providing transformed cell stocks needed to produce the cDNA probes for POMC, proDYN, proENK, and proNPY. This research was supported by General Research Funds of the Department of Veterans Affairs, Minnesota Obesity Center Grant NIDDK DK-50456, and by NIDA Grant DA-03999.

PY - 2000/4/17

Y1 - 2000/4/17

N2 - Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and α-melanocyte stimulating hormone (α-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P>0.05). Obese Zucker rats had lower α-MSH and dynorphin A1-17 peptide levels in the paraventricular nucleus (PVN) (P<0.05), but did not have lower PVN β-endorphin peptide levels (P>0.05). The decrease in POMC in the ARC and decrease in α-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. Copyright (C) 2000.

AB - Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and α-melanocyte stimulating hormone (α-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P>0.05). Obese Zucker rats had lower α-MSH and dynorphin A1-17 peptide levels in the paraventricular nucleus (PVN) (P<0.05), but did not have lower PVN β-endorphin peptide levels (P>0.05). The decrease in POMC in the ARC and decrease in α-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. Copyright (C) 2000.

KW - Energy regulation

KW - Melanocortin

KW - NPY

KW - Opioids

UR - https://www.scopus.com/pages/publications/0034678677

UR - https://www.scopus.com/pages/publications/0034678677#tab=citedBy

U2 - 10.1016/S0006-8993(00)02060-6

DO - 10.1016/S0006-8993(00)02060-6

M3 - Article

C2 - 10799663

AN - SCOPUS:0034678677

SN - 0006-8993

VL - 862

SP - 11

EP - 16

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats

Abstract

Bibliographical note

UN SDGs

Keywords

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