Arachidonic acid metabolism by isolated and cultured middle ear epithelial cells from the chinchilla

T. Harada, S. K. Juhn, Y. Kim, Y. Sakakura

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Thin-layer chromatography was used to examine the metabolism of arachidonic acid and prostaglandins (PGs) in freshly isolated and cultured middle ear epithelial cells from the chinchilla. The freshly isolated cells converted arachidonic acid predominantly to PGE2, while those cells grown in culture for 10 days acquired the ability to convert arachidonic acid to 6-keto-PGF, PGD2, and PGE2. Incubation of the isolated cells and primary cultures with acetylsalicylic acid and indomethacin inhibited the formation of these PGs. These findings suggest that studies on the factors regulating arachidonic acid metabolism in middle ear epithelium may help to explain the role of eicosanoids in middle ear secretions, particularly in relation to the pathophysiology of otitis media.

Original languageEnglish (US)
Pages (from-to)220-223
Number of pages4
JournalOto-Rhino-Laryngology
Volume250
Issue number4
DOIs
StatePublished - Jul 1993

Keywords

  • Arachidonic acid metabolism
  • Chinchilla
  • Epithelial transplants
  • Middle ear

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