Arabidopsis GLABROUS1 gene requires downstream sequences for function

John C. Larkin, David G. Oppenheimer, Susan Pollock, M D Marks

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

The Arabidopsis GLABROUS1 (GL1) gene is a myb gene homolog required for the initiation of trichome development. In situ hybridization revealed that the highest levels of GL1 transcripts were present in developing trichomes. In contrast, previous work had shown that putative promoter sequences from the 5′ noncoding region of the GL1 gene directed the expression of a β-glucuronidase (GUS) reporter gene only in stipules. Deletion analysis of the 3′ noncoding region of GL1 has identified an enhancer that is essential for GL1 function. Sequences from the region containing the enhancer, in conjunction with GL1 upstream sequences, direct the expression of a GUS reporter gene in leaf primordia and developing trichomes in addition to stipules, indicating that the downstream enhancer is required for the normal expression pattern of GL1.

Original languageEnglish (US)
Pages (from-to)1739-1748
Number of pages10
JournalPlant Cell
Volume5
Issue number12
StatePublished - Dec 1 1993

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Trichomes
Arabidopsis
trichomes
Genes
Glucuronidase
Reporter Genes
reporter genes
myb Genes
leaf primordia
genes
in situ hybridization
In Situ Hybridization
promoter regions
Gene Expression

Cite this

Larkin, J. C., Oppenheimer, D. G., Pollock, S., & Marks, M. D. (1993). Arabidopsis GLABROUS1 gene requires downstream sequences for function. Plant Cell, 5(12), 1739-1748.

Arabidopsis GLABROUS1 gene requires downstream sequences for function. / Larkin, John C.; Oppenheimer, David G.; Pollock, Susan; Marks, M D.

In: Plant Cell, Vol. 5, No. 12, 01.12.1993, p. 1739-1748.

Research output: Contribution to journalArticle

Larkin, JC, Oppenheimer, DG, Pollock, S & Marks, MD 1993, 'Arabidopsis GLABROUS1 gene requires downstream sequences for function', Plant Cell, vol. 5, no. 12, pp. 1739-1748.
Larkin JC, Oppenheimer DG, Pollock S, Marks MD. Arabidopsis GLABROUS1 gene requires downstream sequences for function. Plant Cell. 1993 Dec 1;5(12):1739-1748.
Larkin, John C. ; Oppenheimer, David G. ; Pollock, Susan ; Marks, M D. / Arabidopsis GLABROUS1 gene requires downstream sequences for function. In: Plant Cell. 1993 ; Vol. 5, No. 12. pp. 1739-1748.
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