Approaches to the development of assays for interaction of tobacco-specific nitrosamines with haemoglobin and DNA.

S. S. Hecht, S. G. Carmella, N. Trushin, T. E. Spratt, P. G. Foiles, D. Hoffmann

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The tobacco-specific, nicotine-derived nitrosamines 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) are among the most important carcinogens in tobacco and tobacco smoke. Treatment of Fischer 344 rats with these carcinogens resulted in alkylation of haemoglobin and DNA by the 4-(3-pyridyl)-4-oxobutyl group formed during their metabolism. This alkyl group can be detached from globin or DNA under mild hydrolytic conditions as 4-hydroxy-1-(3-pyridyl)-1-butanone, which appears to be a potentially useful dosimeter for human exposure to, and activation of, tobacco-specific nitrosamines.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalIARC scientific publications
Issue number89
StatePublished - Dec 1 1988
Externally publishedYes

Fingerprint Dive into the research topics of 'Approaches to the development of assays for interaction of tobacco-specific nitrosamines with haemoglobin and DNA.'. Together they form a unique fingerprint.

Cite this