Abstract
Differential scanning calorimetry has been investigated for its application to the experimental study of the temperature dependence of the kinetics of enzyme-substrate reactions. A reaction cell was developed which allows mixing of enzyme and substrate solutions directly in the calorimeter. The device was used to study the zero-order reaction of acetylcholinesterase with acetylcholine and the first-order reaction of α-chymotrypsin with N-acetyl-l-alanine methyl ester. The reaction cell is found to be satisfactory in the isothermal mode for both first- and zero-order reactions and in the scanning mode for the zero-order reactions but not for the first-order reaction. Limitations of the design are described for general enzyme kinetic studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7-17 |
| Number of pages | 11 |
| Journal | Thermochimica Acta |
| Volume | 33 |
| Issue number | C |
| DOIs | |
| State | Published - Oct 1979 |