Applications of differential scanning calorimetry to enzyme kinetics

L. F. Whiting, P. W. Carr

Research output: Contribution to journalArticle

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Abstract

Differential scanning calorimetry has been investigated for its application to the experimental study of the temperature dependence of the kinetics of enzyme-substrate reactions. A reaction cell was developed which allows mixing of enzyme and substrate solutions directly in the calorimeter. The device was used to study the zero-order reaction of acetylcholinesterase with acetylcholine and the first-order reaction of α-chymotrypsin with N-acetyl-l-alanine methyl ester. The reaction cell is found to be satisfactory in the isothermal mode for both first- and zero-order reactions and in the scanning mode for the zero-order reactions but not for the first-order reaction. Limitations of the design are described for general enzyme kinetic studies.

Original languageEnglish (US)
Pages (from-to)7-17
Number of pages11
JournalThermochimica Acta
Volume33
Issue numberC
DOIs
StatePublished - Oct 1979

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