Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Michael G. Giacomelli, Kevin J. Chalut, Julie H Ostrander, Adam Wax

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved lowcoherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

Original languageEnglish (US)
Pages (from-to)2452-2454
Number of pages3
JournalOptics Letters
Volume33
Issue number21
DOIs
StatePublished - Nov 1 2008
Externally publishedYes

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