Application of spin labeling to drug assays. I. Synthesis of 2,2,6,6‐tetramethylpiperidin‐4‐one‐1‐oxyl‐15N‐d16

Yul Yost, Carl F. Polnaszek, Ronald P. Mason, Jordan L. Holtzman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Spin labeled drugs can be utilized for immunoassays and determining the fraction of free drug in serum. To improve the sensitivity we have synthesized triacetonamine‐15N‐d17 by cyclization of phorone‐d14 with 15ND3. Phorone‐d14 was obtained by condensation of acetone‐d6. Triacetonamine‐15N‐d17 gave Tempone‐15N‐d16, a nitroxide with just two absorption peaks on the ESR spectrum, whereas Tempone gives 3 peaks. The contribution to line width of methylene and methy1 hydrogens of Tempone is evaluated by appropriate substitution of protium with deuterium.

Original languageEnglish (US)
Pages (from-to)1089-1097
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume18
Issue number8
DOIs
StatePublished - Aug 1981

Keywords

  • Drug Spin Labels
  • Phorone‐d
  • Tempone‐N‐d

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