Application of clustering analyses to the diagnosis of Huntington disease in mice and other diseases with well-defined group boundaries

Jason B. Nikas, Walter C. Low

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Nuclear magnetic resonance (NMR) spectroscopy has emerged as a technology that can provide metabolite information within organ systems in vivo. In this study, we introduced a new method of employing a clustering algorithm to develop a diagnostic model that can differentially diagnose a single unknown subject in a disease with well-defined group boundaries. We used three tests to assess the suitability and the accuracy required for diagnostic purposes of the four clustering algorithms we investigated (K-means, Fuzzy, Hierarchical, and Medoid Partitioning). To accomplish this goal, we studied the striatal metabolomic profile of R6/2 Huntington disease (HD) transgenic mice and that of wild type (WT) mice using high field in vivo proton NMR spectroscopy (9.4. T). We tested all four clustering algorithms (1) with the original R6/2 HD mice and WT mice, (2) with unknown mice, whose status had been determined via genotyping, and (3) with the ability to separate the original R6/2 mice into the two age subgroups (8 and 12 weeks old). Only our diagnostic models that employed ROC-supervised Fuzzy, unsupervised Fuzzy, and ROC-supervised K-means Clustering passed all three stringent tests with 100% accuracy, indicating that they may be used for diagnostic purposes.

Original languageEnglish (US)
Pages (from-to)e133-e147
JournalComputer Methods and Programs in Biomedicine
Volume104
Issue number3
DOIs
StatePublished - Dec 2011

Keywords

  • Clustering analyses
  • Diagnostic methods
  • Fuzzy Clustering
  • Hierarchical Clustering
  • Huntington disease
  • K-means Clustering
  • Medoid Partitioning Clustering
  • Metabolomics
  • Nuclear magnetic resonance spectroscopy
  • Receiver operating characteristic (ROC) curve analysis

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