The oligodendroglial nuclei were purified from mouse brain with more than 97 per cent homogeneity. Cyclic AMP-independent phosphorylation of non-histone protein in oligodendroglial chromatin was studied. Morphine sulfate, in vitro, had no effect on phosphorylation. However, chronic morphine treatment resulted in an increase of phosphorylation in high molecular weight regions of sodium dodecylsulfate (SDS) electrophoresis gel. The increase was not the result of a decrease in phosphoprotein phosphatase activity.
Bibliographical noteFunding Information:
Ackno~lrdyrmenrs~These studies were supported by NIMH Grant DA-00564. Dr. H. H. Loh is a recipient of an NIMH Research Scientist Career Development Award, KZ-DA-70554. The authors wish to express their deepest aooreciation to Mr. Peter Sharp and Dr. H. J. Ralston, Iir’ of the Department of Anatomy. University of California, San Francisco. for their valuable suggestions and technical help in microscopic studies. The authors wish to thank Miss Barbara Halperin for her editorial assistance.
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