Apiaceous vegetables and cruciferous phytochemicals reduced phip-DNA adducts in prostate but not in pancreas of wistar rats

Jae Kyeom Kim, Marissa A. McCormick, Cynthia M Gallaher, Dan D Gallaher, Sabrina P. Trudo

Research output: Contribution to journalArticle

Abstract

We previously showed rats fed with apiaceous vegetables, but not with their putative chemopreventive phytochemicals, reduced colonic DNA adducts formed by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a dietary procarcinogen. We report here the effects of feeding apiaceous and cruciferous vegetables versus their purified predominant phytochemicals, either alone or combined, on prostate and pancreatic PhIP-DNA adduct formation. In experiment I, male Wistar rats received three supplemented diets: CRU (cruciferous vegetables), API (apiaceous vegetables), and CRU+API (both types of vegetables). In experiment II, rats received three diets supplemented with phytochemicals matched to their levels in the vegetables from experiment I: P + I (phenethyl isothiocyanate and indole-3-carbinol), FC (furanocoumarins; 5-methoxypsoralen, 8-methoxypsoralen, and isopimpinellin), and COMBO (P + I and FC combined). After 6 days of feeding, PhIP was injected (10 mg/kg body weight) and animals were killed on day 7. PhIP-DNA adducts were analyzed by LC-MS/MS. In prostate, PhIP-DNA adducts were reduced by API (33%, P < .05), P + I (45%, P < .001), and COMBO (30%, P < .01). There were no effects observed in pancreas. Our results suggest that fresh vegetables and purified phytochemicals lower PhIP-DNA adducts and may influence cancer risk.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalJournal of Medicinal Food
Volume21
Issue number2
DOIs
StatePublished - Jan 1 2018

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DNA Adducts
Phytochemicals
Vegetables
Wistar Rats
Prostate
Pancreas
Diet
Methoxsalen
Body Weight
2-amino-1-methyl-6-phenolimidazo(4,5-b)pyridine-DNA adduct

Keywords

  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
  • DNA adducts
  • apiaceous vegetables
  • cruciferous vegetables
  • heterocyclic aromatic amines

PubMed: MeSH publication types

  • Journal Article

Cite this

Apiaceous vegetables and cruciferous phytochemicals reduced phip-DNA adducts in prostate but not in pancreas of wistar rats. / Kim, Jae Kyeom; McCormick, Marissa A.; Gallaher, Cynthia M; Gallaher, Dan D; Trudo, Sabrina P.

In: Journal of Medicinal Food, Vol. 21, No. 2, 01.01.2018, p. 199-202.

Research output: Contribution to journalArticle

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