Apiaceous vegetable intake modulates expression of DNA damage response genes and microRNA in the rat colon

Jae Kyeom Kim, Daniel D. Gallaher, Sabrina P. Trudo

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We examined chemo-preventive mechanisms of apiaceous vegetable intake against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Rats fed either a purified diet, or purified diet supplemented with 21% apiaceous vegetables (API), were injected with PhIP after 6 d of feeding and colons harvested 24 h later. There was a trend for increased colonic mRNA expression of P-glycoprotein, a PhIP efflux transporter. No DNA damage response genes were up-regulated by API but 13 of these genes were down-regulated, compared to the positive control group, which may have been due to formation of fewer PhIP-DNA adducts. In colonic miRNA profiling, 11 out of 421 miRNAs were modulated in the API group, 3 of which target 19 mRNAs involved in cancer signaling pathways. API increased miR-19a, which targets Ccnd1, a potential oncogene responsible for G1-S cell cycle progression. In conclusion, apiaceous vegetable intake may be chemopreventive through modification of miRNA expression as well as P-glycoprotein expression in colon.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalJournal of Functional Foods
Volume45
DOIs
StatePublished - Jun 2018

Keywords

  • 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
  • Apiaceous vegetables
  • Ccnd1
  • Celery
  • Parsnip
  • microRNA

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