Antisense oligonucleotides against protein kinase CK2-α inhibit growth of squamous cell carcinoma of the head and neck in vitro

Russell A. Faust, Sherif Tawfic, Alan T. Davis, Lori A. Bubash, Khalil Ahmed

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Background: Human squamous cell carninomas of the head and neck (SCCHN) overexpress the protein kinase CK2, and elevated CK2 activity correlates with aggressive tumor behavior and poor clinical outcome. We therefore investigated whether interference with CK2 expression would inhibit SCCHN cell growth in vitro. Methods: We targeted the catalytic (α) subunit of CK2 using an antisense oligodeoxynucleotide (ODN) strategy. Human Ca9-22 cells derived from SCCHN were transfected with CK2-α sense, nonsense, or antisense ODN; CK2 activity was measured; and the effect on CK2 activity and on cell growth was determined. Results: Transfection of Ca9-22 cells with antisense CK2-α ODN resulted in significantly decreased CK2 kinase activity associated with nuclear chromatin and in dose-dependent growth inhibition of Ca9-22 cells in vitro. Conclusions: Interference with the protein kinase CK2 signal in SCCHN cells may offer a novel anticancer strategy for this malignancy. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalHead and Neck
Volume22
Issue number4
DOIs
StatePublished - Jul 2000

Keywords

  • Chromatin
  • Gene therapy
  • Kinase
  • Nuclear matrix
  • Oncogene
  • Oncoprotein
  • Prognostic marker

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