Antisem to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines

Keith M Skubitz; Thomas P. Ducker; Amy P Skubitz; Said A. Goueli

doi:10.1016/0014-5793(93)80021-L

Antisem to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines

Keith M Skubitz, Thomas P. Ducker, Amy P Skubitz, Said A. Goueli

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [γ-³²P]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175-190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175-190 kDa protein showed that it contained predominantly phosphotyrosine.

Original language	English (US)
Pages (from-to)	200-204
Number of pages	5
Journal	FEBS Letters
Volume	318
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)80021-L
State	Published - Mar 1 1993

Bibliographical note

Funding Information:
AcknowledgemenrsW: e thankK . Campbellf or excellentte chnicaal s-sistanceD, rs. S. Hefta and R. Paxtonf or providingm onoclonaal nti-bodies,D r. K. Ahmed for a criticalr eviewo f the manuscripta, nd C. Stockef or manuscrippt reparationT. his work was supportedin part by the AmericanH eart Association,t he LeukemiaT ask Force, the Universityo f MinnesotaG raduateS chool.a nd the MasomcM emo-rial Hospital Fund, Inc.

Keywords

Biliary glycoprotein
Carcinoembryonic antigen
Ectoprotein kinase
Phosphotyrosine
Protein phosphorylation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(93)80021-L

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title = "Antisem to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines",

abstract = "Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [γ-32P]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175-190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175-190 kDa protein showed that it contained predominantly phosphotyrosine.",

keywords = "Biliary glycoprotein, Carcinoembryonic antigen, Ectoprotein kinase, Phosphotyrosine, Protein phosphorylation",

author = "Skubitz, {Keith M} and Ducker, {Thomas P.} and Skubitz, {Amy P} and Goueli, {Said A.}",

note = "Funding Information: AcknowledgemenrsW: e thankK . Campbellf or excellentte chnicaal s-sistanceD, rs. S. Hefta and R. Paxtonf or providingm onoclonaal nti-bodies,D r. K. Ahmed for a criticalr eviewo f the manuscripta, nd C. Stockef or manuscrippt reparationT. his work was supportedin part by the AmericanH eart Association,t he LeukemiaT ask Force, the Universityo f MinnesotaG raduateS chool.a nd the MasomcM emo-rial Hospital Fund, Inc.",

year = "1993",

month = mar,

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doi = "10.1016/0014-5793(93)80021-L",

language = "English (US)",

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T1 - Antisem to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines

AU - Skubitz, Keith M

AU - Ducker, Thomas P.

AU - Skubitz, Amy P

AU - Goueli, Said A.

N1 - Funding Information: AcknowledgemenrsW: e thankK . Campbellf or excellentte chnicaal s-sistanceD, rs. S. Hefta and R. Paxtonf or providingm onoclonaal nti-bodies,D r. K. Ahmed for a criticalr eviewo f the manuscripta, nd C. Stockef or manuscrippt reparationT. his work was supportedin part by the AmericanH eart Association,t he LeukemiaT ask Force, the Universityo f MinnesotaG raduateS chool.a nd the MasomcM emo-rial Hospital Fund, Inc.

PY - 1993/3/1

Y1 - 1993/3/1

N2 - Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [γ-32P]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175-190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175-190 kDa protein showed that it contained predominantly phosphotyrosine.

AB - Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [γ-32P]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175-190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175-190 kDa protein showed that it contained predominantly phosphotyrosine.

KW - Biliary glycoprotein

KW - Carcinoembryonic antigen

KW - Ectoprotein kinase

KW - Phosphotyrosine

KW - Protein phosphorylation

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ER -

Antisem to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines

Abstract

Bibliographical note

Keywords

UN SDGs

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