The present study is an extension of our previous research work and it deals with the scientific evaluation of antioxidant potential of the aqueous extract of Cynodon dactylon. on diabetes-induced oxidative stress of diabetic rats. The most effective dose of 500 mg/kg of extract was given orally to diabetic rats for 30 days. Different oxidative stress parameters were analyzed in various tissues of control and treated diabetic rats. The observed elevated level of LPO comes down significantly (P < 0.05), and decreased activities of antioxidant enzymes such as CAT, SOD, GPx and GST got increased (P < 0.05) significantly in diabetic rats on extract treatment. The flavonoids present in the aqueous extract of this plant might be responsible for its marked antioxidant efficacy at tissue level in STZ-induced diabetic rats.