Antimicrobial activity of triantibiotic paste, 2% chlorhexidine gel, and calcium hydroxide on an intraoral-infected dentin biofilm model

Ronald Ordinola-Zapata, Clovis M. Bramante, Paloma Gagliardi Minotti, Bruno Cavalini Cavenago, Roberto Brandão Garcia, Norberti Bernardineli, David E. Jaramillo, Marco A. Hungaro Duarte

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Introduction: The purpose of this study was to evaluate the antimicrobial activity of calcium hydroxide, 2% chlorhexidine gel, and triantibiotic paste (ie, metronidazole, minocycline, and ciprofloxacin) by using an intraorally infected dentin biofilm model. Methods: Forty bovine dentin specimens were infected intraorally using a removable orthodontic device in order to induce the biofilm colonization of the dentin. Then, the samples were treated with the medications for 7 days. Saline solution was used as the control. Two evaluations were performed: immediately after the elimination of the medication and after incubation in brain-heart infusion medium for 24 hours. The Live/Dead technique (Invitrogen, Eugene, OR) and a confocal microscope were used to obtain the percentage of live cells. Nonparametric statistical tests were performed to show differences in the percentage of live cells among the groups (P <.05). Results: Calcium hydroxide and 2% chlorhexidine gel did not show statistical differences in the immediate evaluation. However, after application of the brain-heart infusion medium for 24 hours, 2% gel chlorhexidine showed a statistically lesser percentage of live cells in comparison with calcium hydroxide. The triantibiotic paste significantly showed a lower percentage of live cells in comparison with the 2% chlorhexidine gel and calcium hydroxide groups in the immediate and secondary (after 24 hours) evaluations. Conclusions: The triantibiotic paste was most effective at killing the bacteria in the biofilms on the intraorally infected dentin model in comparison with 2% chlorhexidine gel and calcium hydroxide.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalJournal of Endodontics
Volume39
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Keywords

  • Antimicrobials
  • bacteria
  • biofilms
  • dentin
  • root canal dressings

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