Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms

M. T. Arias-Moliz, R. Ordinola-Zapata, P. Baca, M. Ruiz-Linares, E. García García, M. A. Hungaro Duarte, C. Monteiro Bramante, C. M. Ferrer-Luque

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Aim: To evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of Enterococcus faecalis biofilms attached to dentine. Methodology: Biofilms of E. faecalis were grown on the surface of dentine blocks for 5 days and then exposed to the irrigating solutions for 3 min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P < 0.05). Results: NaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (P < 0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups. Conclusions: NaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E. faecalis biofilms.

Original languageEnglish (US)
Pages (from-to)1188-1193
Number of pages6
JournalInternational Endodontic Journal
Volume48
Issue number12
DOIs
StatePublished - Dec 2015
Externally publishedYes

Keywords

  • Antimicrobials
  • Bacteria
  • Dentine infection
  • Root canal irrigants

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