Antigenic variation in visna virus

Jane V. Scott, Linda Stowring, Ashley T. Haase, Opendra Narayan, Robert Vigne

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Two antigenic variants of visna virus were isolated sequentially from a single sheep inoculated with a plaque-purified strain of virus designated 1514. The genetically stable variants, LV1-1 and LV1-4, are of two classes: LV1-1 is partially neutralized by antibody to the inoculum strain 1514, while LV1-4 is not neutralized by antibody to 1514. The genetic mechanism responsible for generating the antigenic variants was investigated by comparing the chymotryptic and tryptic maps of the envelope glycoprotein gp135 and core polypeptides (p30, p16, p14), and by comparing the pattern of large oligonucleotides produced by digestion of the RNAs by T1 ribonuclease. We show that only the peptide maps of gp135 differ among strains, that the number of peptide fragments altered is small and that gp135 is the polypeptide that elicits neutralizing antibody. The maps of the RNAs are identical. We conclude that mutation in the glycoprotein gene rather than recombination is more probably responsible for antigenic variation, and speculate on the special aspects of visna virus replication relevant to this phenomenon.

Original languageEnglish (US)
Pages (from-to)321-327
Number of pages7
JournalCell
Volume18
Issue number2
DOIs
StatePublished - Oct 1979

Fingerprint Dive into the research topics of 'Antigenic variation in visna virus'. Together they form a unique fingerprint.

Cite this