Antigenic diversity of granulocytic Ehrlichia isolates from humans in Wisconsin and New York and a horse in California

Kristin M. Asanovich, Johan S. Bakken, John E. Madigan, Maria Aguero-Rosenfeld, Gary P. Wormser, J. Stephen Dumler

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

The agent of human granulocytic ehrlichiosis (HGE), Ehrlichia phagocytophila, and Ehrlichia equi are very similar. HGE is of variable severity. Genetic and antigenic differences among 3 human isolates (Webster, Spooner, and NY-8) and 1 horse isolate (MRK) were evaluated. The 16S rRNA gene sequences were identical in all human isolates. By use of 5 homologous antisera from these 3 humans and 1 horse and an additional 5 antisera in heterologous reactions, the immunodominant antigens of each isolate were noted to differ in molecular size: 43 kDa in the Webster (Wisconsin) isolate, 46 kDa in the Spooner (Wisconsin) isolate, 42 and 45 kDa in the NY-8 (New York State) isolate, and a 42 kDa doublet in the E. equi MRK isolate from California. Two sera from a Wisconsin patient reacted weakly or not at all with the NY-8 isolate. Antigenic structural diversity exists among otherwise indistinguishable granulocytic ehrlichial isolates.

Original languageEnglish (US)
Pages (from-to)1029-1034
Number of pages6
JournalJournal of Infectious Diseases
Volume176
Issue number4
DOIs
StatePublished - 1997

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