Antifungal Norditerpene Oidiolactones from the Fungus Oidiodendron truncatum, a Potential Biocontrol Agent for White-Nose Syndrome in Bats

Yudi Rusman, Michael B. Wilson, Jessica M. Williams, Benjamin W. Held, Robert A. Blanchette, Brianna N. Anderson, Christopher R. Lupfer, Christine E. Salomon

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

White-nose syndrome (WNS) is a devastating disease of hibernating bats caused by the fungus Pseudogymnoascus destructans. We obtained 383 fungal and bacterial isolates from the Soudan Iron Mine, an important bat hibernaculum in Minnesota, then screened this library for antifungal activity to develop biological control treatments for WNS. An extract from the fungus Oidiodendron truncatum was subjected to bioassay-guided fractionation, which led to the isolation of 14 norditerpene and three anthraquinone metabolites. Ten of these compounds were previously described in the literature, and here we present the structures of seven new norditerpene analogues. Additionally, this is the first report of 4-chlorophyscion from a natural source, previously identified as a semisynthetic product. The compounds PR 1388 and LL-Z1271α were the only inhibitors of P. destructans (MIC = 7.5 and 15 μg/mL, respectively). Compounds were tested for cytotoxicity against fibroblast cell cultures obtained from Myotis septentrionalis (northern long eared bat) and M. grisescens (gray bat) using a standard MTT viability assay. The most active antifungal compound, PR 1388, was nontoxic toward cells from both bat species (IC50 > 100 μM). We discuss the implications of these results in the context of the challenges and logistics of developing a substrate treatment or prophylactic for WNS.

Original languageEnglish (US)
Pages (from-to)344-353
Number of pages10
JournalJournal of Natural Products
Volume83
Issue number2
DOIs
StatePublished - Feb 28 2020

Bibliographical note

Funding Information:
This work was funded by grant ML 2016 Chp 186, sec 2, from the Environmental and Natural Resources Trust Fund by the State of Minnesota to C.E.S. and R.A.B. and USFW F15AP01056 to C.E.S. and R.A.B. We thank J. Nugent for the human fibroblast cell line culture and G. Krause for assistance with the fungal assays. We are grateful to J. Essig, J. Pointer, and the Soudan Underground Mine State Park staff for facilitating field collections. We thank G. Nordquist for helpful discussions and field assistance and the Minnesota Department of Natural Resources for permits and logistical support.

Publisher Copyright:
Copyright © 2020 American Chemical Society and American Society of Pharmacognosy.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Fingerprint

Dive into the research topics of 'Antifungal Norditerpene Oidiolactones from the Fungus Oidiodendron truncatum, a Potential Biocontrol Agent for White-Nose Syndrome in Bats'. Together they form a unique fingerprint.

Cite this