TY - JOUR
T1 - Antibody-dependent Lymphocyte-mediated Granulocytotoxicity (ADLG) for the detection of granulocyte antibodies
AU - Richards, Karen
AU - Sayed Mohammad Hossein Sadrzadeh, Mohammad Hossein Sadrzadeh
AU - Clay, Mary
AU - Mc Cullough, Jeffrey
PY - 1983/9/30
Y1 - 1983/9/30
N2 - An antibody-dependent cell-mediated granulocytotoxic assay (ADLG) has been developed to detect antibodies to human granulocytes. This study examines some possible variables in this assay, the ADLG activity in serum from normal individuals, the contribution of ABO isohemagglutinins and the sensitivity and specificity of this technique. Significant differences in percentage cytotoxicity of ABO compatible and incompatible cell/serum combinations were detected in the normal population. Human serum with antibodies to the neutrophil specific antigens NA1 and NB1 gave positive results in the ADLG assay. However, differences in sensitivity and specificity were observed among various sera. ADLG activity was dependent on the source of effector cells. Effector and target cells stored in vitro were unsatisfactory in this assay. The ADLG assay may be a valuable addition to existing granulocyte serological methods and provide useful clinical information on mechanisms of immune neutropenias.
AB - An antibody-dependent cell-mediated granulocytotoxic assay (ADLG) has been developed to detect antibodies to human granulocytes. This study examines some possible variables in this assay, the ADLG activity in serum from normal individuals, the contribution of ABO isohemagglutinins and the sensitivity and specificity of this technique. Significant differences in percentage cytotoxicity of ABO compatible and incompatible cell/serum combinations were detected in the normal population. Human serum with antibodies to the neutrophil specific antigens NA1 and NB1 gave positive results in the ADLG assay. However, differences in sensitivity and specificity were observed among various sera. ADLG activity was dependent on the source of effector cells. Effector and target cells stored in vitro were unsatisfactory in this assay. The ADLG assay may be a valuable addition to existing granulocyte serological methods and provide useful clinical information on mechanisms of immune neutropenias.
KW - antibody-dependent cytotoxicity
KW - granulocytes
KW - neutrophil antibodies
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U2 - 10.1016/0022-1759(83)90212-0
DO - 10.1016/0022-1759(83)90212-0
M3 - Article
C2 - 6619551
AN - SCOPUS:0021035167
SN - 0022-1759
VL - 63
SP - 93
EP - 102
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -