Antibodies to endogenous tear protein in normal human tears

Susann G. Remington; Jean M. Crow; J. Daniel Nelson

doi:10.3109/02713680903108531

Antibodies to endogenous tear protein in normal human tears

Susann G. Remington, Jean M. Crow, J. Daniel Nelson

Ophthalmology & Visual Neurosciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Purpose: To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears. Materials and Methods: Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins. Results: Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins. Conclusion: Normal human ocular tears contain antibodies to endogenous tear protein.

Original language	English (US)
Pages (from-to)	819-823
Number of pages	5
Journal	Current Eye Research
Volume	34
Issue number	10
DOIs	https://doi.org/10.3109/02713680903108531
State	Published - Oct 2009

Bibliographical note

Funding Information:
This work was supported by a grant from HealthPart-ners Research Foundation, Minneapolis, Minnesota.

Keywords

Autoantibodies
Lipocalin-1
Lysozyme
Ocular tears

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10.3109/02713680903108531

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title = "Antibodies to endogenous tear protein in normal human tears",

abstract = "Purpose: To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears. Materials and Methods: Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins. Results: Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins. Conclusion: Normal human ocular tears contain antibodies to endogenous tear protein.",

keywords = "Autoantibodies, Lipocalin-1, Lysozyme, Ocular tears",

author = "Remington, {Susann G.} and Crow, {Jean M.} and Nelson, {J. Daniel}",

note = "Funding Information: This work was supported by a grant from HealthPart-ners Research Foundation, Minneapolis, Minnesota.",

year = "2009",

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doi = "10.3109/02713680903108531",

language = "English (US)",

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TY - JOUR

T1 - Antibodies to endogenous tear protein in normal human tears

AU - Remington, Susann G.

AU - Crow, Jean M.

AU - Nelson, J. Daniel

N1 - Funding Information: This work was supported by a grant from HealthPart-ners Research Foundation, Minneapolis, Minnesota.

PY - 2009/10

Y1 - 2009/10

N2 - Purpose: To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears. Materials and Methods: Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins. Results: Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins. Conclusion: Normal human ocular tears contain antibodies to endogenous tear protein.

AB - Purpose: To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears. Materials and Methods: Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins. Results: Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins. Conclusion: Normal human ocular tears contain antibodies to endogenous tear protein.

KW - Autoantibodies

KW - Lipocalin-1

KW - Lysozyme

KW - Ocular tears

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ER -

Antibodies to endogenous tear protein in normal human tears

Abstract

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