Antibiotic treatment disrupts bacterial communities in the colon and rectum of simian immunodeficiency virus-infected macaques

Alexander S Zevin, Tiffany Hensley-McBain, Charlene Miller, Elise Smith, Stanley Langevin, Nichole R Klatt

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected colon biopsies, stool samples and rectal swabs before and after antibiotics, and evaluated the bacterial communities at each sample site using high-throughput 16S rRNA gene sequencing. The colon mucosa and stool samples displayed different bacterial communities, while the rectal swabs showed a mixture of the mucosal and stool-associated bacteria. Antibiotics disrupted the native bacterial communities at each sample site. The colon mucosa showed depleted abundances of the dominant Helicobacteraceae, while we found depleted abundances of the dominant Ruminococcaceae sp. in the stool. The rectal swabs showed similar trends as the colon mucosa, but were more variable. After the antibiotic treatment, there were increased abundances of similar taxa of facultative anaerobic bacteria, including Lactobacillaceae and Enterobacteriaceae at each sample site.

Original languageEnglish (US)
JournalFEMS Microbiology Letters
Volume364
Issue number23
DOIs
StatePublished - Dec 15 2017

Bibliographical note

© FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Keywords

  • Animals
  • Anti-Bacterial Agents/pharmacology
  • Bacterial Load/drug effects
  • Colon/microbiology
  • Feces/microbiology
  • Gastrointestinal Microbiome/drug effects
  • Macaca mulatta
  • Male
  • Rectum/microbiology
  • Simian Acquired Immunodeficiency Syndrome/microbiology

PubMed: MeSH publication types

  • Journal Article

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