Antibacterial activity of a glass ionomer cement doped with copper nanoparticles

David Aguilar-Perez; Rossana Vargas-Coronado; Jose M. Cervantes-Uc; Nayeli Rodriguez-Fuentes; Conrado Aparicio; Cristian Covarrubias; Marco Alvarez-Perez; Victor Garcia-Perez; Miryam Martinez-Hernandez; Juan Valerio Cauich-Rodriguez

doi:10.4012/dmj.2019-046

Antibacterial activity of a glass ionomer cement doped with copper nanoparticles

David Aguilar-Perez, Rossana Vargas-Coronado, Jose M. Cervantes-Uc, Nayeli Rodriguez-Fuentes, Conrado Aparicio, Cristian Covarrubias, Marco Alvarez-Perez, Victor Garcia-Perez, Miryam Martinez-Hernandez, Juan Valerio Cauich-Rodriguez

Biomaterials

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (Cu₂O). HDPF viability reduces with exposure time to the extracts (68–72% viability) and MGIC with 2–4 wt% NCu showed antimicrobial activity against the two tested strains.

Original language	English (US)
Article number	dmj/2019-046
Pages (from-to)	389-396
Number of pages	8
Journal	Dental Materials Journal
Volume	39
Issue number	3
DOIs	https://doi.org/10.4012/dmj.2019-046
State	Published - 2020

Bibliographical note

Funding Information:
This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Treviño and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments.

Funding Information:
This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Trevi?o and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments.

Publisher Copyright:
© 2020, Japanese Society for Dental Materials and Devices. All rights reserved.

Keywords

Anaerobia bacteria
Biomaterial
Dental cement
Fibroblast
Metal nanoparticle

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10.4012/dmj.2019-046

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Aguilar-Perez, D., Vargas-Coronado, R., Cervantes-Uc, J. M., Rodriguez-Fuentes, N., Aparicio, C., Covarrubias, C., Alvarez-Perez, M., Garcia-Perez, V., Martinez-Hernandez, M., & Cauich-Rodriguez, J. V. (2020). Antibacterial activity of a glass ionomer cement doped with copper nanoparticles. Dental Materials Journal, 39(3), 389-396. Article dmj/2019-046. https://doi.org/10.4012/dmj.2019-046

Aguilar-Perez, D, Vargas-Coronado, R, Cervantes-Uc, JM, Rodriguez-Fuentes, N, Aparicio, C, Covarrubias, C, Alvarez-Perez, M, Garcia-Perez, V, Martinez-Hernandez, M & Cauich-Rodriguez, JV 2020, 'Antibacterial activity of a glass ionomer cement doped with copper nanoparticles', Dental Materials Journal, vol. 39, no. 3, dmj/2019-046, pp. 389-396. https://doi.org/10.4012/dmj.2019-046

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title = "Antibacterial activity of a glass ionomer cement doped with copper nanoparticles",

abstract = "Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (Cu2O). HDPF viability reduces with exposure time to the extracts (68–72% viability) and MGIC with 2–4 wt% NCu showed antimicrobial activity against the two tested strains.",

keywords = "Anaerobia bacteria, Biomaterial, Dental cement, Fibroblast, Metal nanoparticle",

author = "David Aguilar-Perez and Rossana Vargas-Coronado and Cervantes-Uc, {Jose M.} and Nayeli Rodriguez-Fuentes and Conrado Aparicio and Cristian Covarrubias and Marco Alvarez-Perez and Victor Garcia-Perez and Miryam Martinez-Hernandez and Cauich-Rodriguez, {Juan Valerio}",

note = "Funding Information: This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Trevi{\~n}o and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments. Funding Information: This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Trevi?o and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments. Publisher Copyright: {\textcopyright} 2020, Japanese Society for Dental Materials and Devices. All rights reserved.",

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doi = "10.4012/dmj.2019-046",

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AU - Aguilar-Perez, David

AU - Vargas-Coronado, Rossana

AU - Cervantes-Uc, Jose M.

AU - Rodriguez-Fuentes, Nayeli

AU - Aparicio, Conrado

AU - Covarrubias, Cristian

AU - Alvarez-Perez, Marco

AU - Garcia-Perez, Victor

AU - Martinez-Hernandez, Miryam

AU - Cauich-Rodriguez, Juan Valerio

N1 - Funding Information: This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Treviño and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments. Funding Information: This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Trevi?o and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments. Publisher Copyright: © 2020, Japanese Society for Dental Materials and Devices. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (Cu2O). HDPF viability reduces with exposure time to the extracts (68–72% viability) and MGIC with 2–4 wt% NCu showed antimicrobial activity against the two tested strains.

AB - Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (Cu2O). HDPF viability reduces with exposure time to the extracts (68–72% viability) and MGIC with 2–4 wt% NCu showed antimicrobial activity against the two tested strains.

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KW - Biomaterial

KW - Dental cement

KW - Fibroblast

KW - Metal nanoparticle

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Antibacterial activity of a glass ionomer cement doped with copper nanoparticles

Abstract

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Keywords

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