The pro-inflammatory and anti-inflammatory maladjustment has been acknowledged as one of the chief causations of inflammatory diseases and even cancers. Previous studies showed that plant-derived polyphenolic compounds were the most potent anti-oxidant and anti-inflammatory agents among all natural compounds. The present study indicates that bound polyphenols of inner shell (BPIS) from foxtail millet bran can display anti-inflammatory effects in LPS-induced HT-29 cells and in nude mice. Mechanistically, BPIS restrained the level of various pro-inflammatory cytokines (IL-1β, IL-6, IL-8), and enhanced the expression level of anti-inflammatory cytokine (IL-10) by blocking the nuclear factor-kappaB (NF- κB)-p65 nuclear translocation. Further, we found the elevated miR-149 expression by BPIS-induced ROS accumulation, directly targeted the Akt expression to block NF-κB nuclear translocation. Taken together, these novel findings provide new insights into the development of BPIS as an anti-inflammatory agent via the signaling cascade of ROS/miR-149/Akt/NF-κB axis.
Bibliographical noteFunding Information:
This study is supported by the National Natural Science Foundation of China No. 31500630), National Training Program of Innovation and Entrepreneurship for Undergraduates (201610119005). Research project supported by Shanxi Scholarship Council of China. Shanxi Province Science Foundation for Youths (2015021200), Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi (2015175).
© Shi et al.
- Bound polyphenols
- Foxtail millet bran