Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells

Hye Ja Lee, Gyeoung Jin Kang, Weon Jong Yoon, Hee Kyoung Kang, Young Suk Kim, So Mi Kim, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform (CHCl3) fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of 100 μg/ml in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of 50 μg/ml in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalKorean Journal of Pharmacognosy
Volume37
Issue number2
StatePublished - Jun 1 2006

Keywords

  • COX
  • Citrus grandis Osbeck
  • IL-6
  • MDC
  • TARC
  • iNOS

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