Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells

Weon Jong Yoon, Soo Jin Heo, Sang Chul Han, Hye Ja Lee, Gyeoung Jin Kang, Hee Kyoung Kang, Jin Won Hyun, Young Sang Koh, Eun Sook Yoo

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45 Scopus citations

Abstract

A study on the anti-inflammatory activity of brown alga Sargassum siliquastrum led to the isolation of sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dosedependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPSinduced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation.

Original languageEnglish (US)
Pages (from-to)1421-1430
Number of pages10
JournalArchives of Pharmacal Research
Volume35
Issue number8
DOIs
StatePublished - Aug 2012

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF-C1-2011-0021039)

Keywords

  • Inflammatory markers
  • MAPK
  • NF-κB
  • Pro-inflammatory cytokines
  • Sargachromanol G
  • Sargassum siliquastrum

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