Anti-Inflammatory effect of Dangyuja (Citrus grandis osbeck) Leaves in LPS-Stimulated RAW 264.7 Cells

Eun Jin Yang, Hye Ja Lee, Gyeoung Jin Kang, Sun Soon Park, Weon Jong Yoon, Hee Kyoung Kang, Somi Kim Cho, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Dangyuja (Citrus grandis Osbeck) is a native plant growing only on Jeju Island in Korea. In this study, antiinflammatory effect of dangyuja leaves on a murine macrophage cell line was investigated. RAW 264.7 murine macrophage cells were stimulated with lipopolysaccharide (LPS, 1 μg/mL) to induce expression of pro-inflammatory markers [interleukin (IL)-6 and inducible nitric oxide synthase (iNOS)]. The crude extract (80% MeOH Ex.) and solvent fractions (hexane, CHCl3, EtOAc, BuOH, and H2O Ex.) were obtained from dangyuja leaves. The CHCl3 fraction inhibited the nitric oxide (NO) and IL- 6 production in a dose-dependent manner. Also, the CHCl3 fraction inhibited mRNA expression and protein levels of iNOS in a dose-dependent manner. Furthermore, the CHCl3 fraction inhibited LPS-induced nuclear factor (NF)-κB activation and phosphorylation of mitogen-activated protein kinases (MAPKs: ERK, JNK, and p38). These results suggest that dangyuja leaves may inhibit LPS-induced production of inflammatory markers by blocking NF-κB and MAPKs signaling in RAW 264.7 cells.

Original languageEnglish (US)
Pages (from-to)1063-1070
Number of pages8
JournalFood Science and Biotechnology
Issue number5
StatePublished - Oct 2009


  • Dangyuja (citrus grandis osbeck)
  • Inflammation
  • Inflammatory marker
  • Mitogen-activated protein kinases (mapks)
  • Nuclear factor κb


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