Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Ryota Sakamoto, Vincent Noireaux, Yusuke T. Maeda

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

Original languageEnglish (US)
Article number7364
JournalScientific reports
Issue number1
StatePublished - Dec 1 2018

Bibliographical note

Publisher Copyright:
© 2018 The Author(s).


Dive into the research topics of 'Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions'. Together they form a unique fingerprint.

Cite this