Anaplastic large cell lymphoma: A flow cytometric analysis of 29 cases

Melissa V. Kesler, Geeta S. Paranjape, Sheryl L. Asplund, Robert W. McKenna, Saba Jamal, Steven H. Kroft

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

We report our experience with flow cytometric (FC) analysis of 29 cases of anaplastic large cell lymphoma (ALCL). Morphologic analysis of processed cytocentrifuged preparations demonstrated neoplastic cells in 28 cases. In 25 of these, an aberrant lymphoid population was detected by FC analysis. The majority showed high orthogonal light scatter, similar to monocytes or granulocytes. Of the antigens CD2, CD3, CD4, CD5, and CD7, 5 cases expressed 1, 8 expressed 2, 6 expressed 3, 3 expressed 4, and 3 expressed all 5. CD4 was expressed most commonly (20/25 [80%]), followed by CD2 (18/25 [72%]), CD3 (10/25 [40%]), and CD5 and CD7 (8/25 [32%] each). CD45 was expressed in 23 of 25 cases and CD13 in 7 of 9. Of 21 cases, 13 were anaplastic lymphoma kinase (ALK)+, all of which were CD4+, vs 5 of 8 ALK- cases (P = .042). Most ALCLs can be detected and characterized by multiparameter FC analysis. However, light scatter gating on typical lymphoid regions may yield false-negative results in a substantial number of cases.

Original languageEnglish (US)
Pages (from-to)314-322
Number of pages9
JournalAmerican journal of clinical pathology
Volume128
Issue number2
DOIs
StatePublished - Aug 2007

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • ALK
  • Anaplastic large cell lymphoma
  • Anaplastic lymphoma kinase
  • Flow cytometry

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