Anaplasma phagocytophilum infection induces apoptosis in HL-60 cells

Pratap Karki, Jacob W. IJdo

Research output: Contribution to journalArticlepeer-review

Abstract

Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intracellular bacterium that survives in neutrophils by delaying apoptosis. The human promyelocytic leukemia cell line HL-60 has been the ultimate choice for culturing Anaplasma in vitro. In this study, we assessed the various events of drug-induced apoptosis in A. phagocytophilum-infected HL-60 cells. Anaplasma infection reduced the cell viability and increased the apoptosis in HL-60 cells and staurosporine or etoposide-induced apoptosis was further exacerbated with Anaplasma infection. Altogether our results suggest that A. phagocytophilum infection is proapoptotic in HL-60 cells unlike in neutrophils where it is antiapoptotic.

Original languageEnglish (US)
Pages (from-to)2741-2746
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Volume27
Issue number11
DOIs
StatePublished - Nov 2011
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments This study was supported in part by a grant from National Institute of Health (RO1AI076244) to JWI. We would like to thank Mark Ver Meer and Elizabeth L. Kennedy for their helpful discussions.

Keywords

  • Anaplasma
  • Anaplasmosis
  • Apoptosis
  • HL-60
  • Neutrophils

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