Abstract
Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 110-119 |
| Number of pages | 10 |
| Journal | Ticks and Tick-borne Diseases |
| Volume | 4 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Feb 1 2013 |
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Keywords
- Anaplasma
- Cervid
- Novel species
- White-tailed deer
Cite this
Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus). / Tate, Cynthia M.; Howerth, Elizabeth W.; Mead, Daniel G.; Dugan, Vivien G.; Luttrell, M. Page; Sahora, Alexandra I.; Munderloh, Ulrike G; Davidson, William R.; Yabsley, Michael J.
In: Ticks and Tick-borne Diseases, Vol. 4, No. 1-2, 01.02.2013, p. 110-119.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus)
AU - Tate, Cynthia M.
AU - Howerth, Elizabeth W.
AU - Mead, Daniel G.
AU - Dugan, Vivien G.
AU - Luttrell, M. Page
AU - Sahora, Alexandra I.
AU - Munderloh, Ulrike G
AU - Davidson, William R.
AU - Yabsley, Michael J.
PY - 2013/2/1
Y1 - 2013/2/1
N2 - Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain.
AB - Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain.
KW - Anaplasma
KW - Cervid
KW - Novel species
KW - White-tailed deer
UR - http://www.scopus.com/inward/record.url?scp=84872617647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872617647&partnerID=8YFLogxK
U2 - 10.1016/j.ttbdis.2012.09.005
DO - 10.1016/j.ttbdis.2012.09.005
M3 - Article
VL - 4
SP - 110
EP - 119
JO - Ticks and Tick-borne Diseases
JF - Ticks and Tick-borne Diseases
SN - 1877-959X
IS - 1-2
ER -