Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus)

Cynthia M. Tate, Elizabeth W. Howerth, Daniel G. Mead, Vivien G. Dugan, M. Page Luttrell, Alexandra I. Sahora, Ulrike G Munderloh, William R. Davidson, Michael J. Yabsley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain.

Original languageEnglish (US)
Pages (from-to)110-119
Number of pages10
JournalTicks and Tick-borne Diseases
Volume4
Issue number1-2
DOIs
StatePublished - Feb 1 2013

Fingerprint

Anaplasmataceae
Anaplasma
Odocoileus
Deer
Odocoileus virginianus
deer
new species
Ticks
ticks
Blood Platelets
Anaplasma platys
Ehrlichia
Amblyomma americanum
fawns
Antibodies
thrombocytopenia
blood
organisms
antibody detection
Transmission Electron Microscopy

Keywords

  • Anaplasma
  • Cervid
  • Novel species
  • White-tailed deer

Cite this

Tate, C. M., Howerth, E. W., Mead, D. G., Dugan, V. G., Luttrell, M. P., Sahora, A. I., ... Yabsley, M. J. (2013). Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus). Ticks and Tick-borne Diseases, 4(1-2), 110-119. https://doi.org/10.1016/j.ttbdis.2012.09.005

Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus). / Tate, Cynthia M.; Howerth, Elizabeth W.; Mead, Daniel G.; Dugan, Vivien G.; Luttrell, M. Page; Sahora, Alexandra I.; Munderloh, Ulrike G; Davidson, William R.; Yabsley, Michael J.

In: Ticks and Tick-borne Diseases, Vol. 4, No. 1-2, 01.02.2013, p. 110-119.

Research output: Contribution to journalArticle

Tate, CM, Howerth, EW, Mead, DG, Dugan, VG, Luttrell, MP, Sahora, AI, Munderloh, UG, Davidson, WR & Yabsley, MJ 2013, 'Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus)', Ticks and Tick-borne Diseases, vol. 4, no. 1-2, pp. 110-119. https://doi.org/10.1016/j.ttbdis.2012.09.005
Tate, Cynthia M. ; Howerth, Elizabeth W. ; Mead, Daniel G. ; Dugan, Vivien G. ; Luttrell, M. Page ; Sahora, Alexandra I. ; Munderloh, Ulrike G ; Davidson, William R. ; Yabsley, Michael J. / Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus). In: Ticks and Tick-borne Diseases. 2013 ; Vol. 4, No. 1-2. pp. 110-119.
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