Analyzing the meiotic transcriptome using isolated meiocytes of arabidopsis thalianaanalyzing the meiotic transcriptome using isolated meiocytes of arabidopsis thaliana

Changbin Chen, Ernest F. Retzel

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

Improved transcriptome sequencing technologies (RNA-seq) have advanced our understanding of the tissue-speci fi c transcriptome landscapes, including those of messenger RNAs, noncoding RNAs and small RNAs. However, transcriptome pro fi les of plant meiocytes remain challenging due to the lack of ef fi cient methods to enrich meiocytes for the analysis of temporal and spatial gene expression patterns during meiosis. In this chapter, we describe a method to analyze the Arabidopsis meiotic transcriptome using isolated male meiocytes.

Original languageEnglish (US)
Title of host publicationPlant Meiosis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages203-213
Number of pages11
ISBN (Print)9781627033329
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume990
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • Capillary collection method
  • Meiocytes
  • RNA-seq
  • Transcriptome

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