Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast

Dong Hwan Kim, Deanna M. Koepp

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cell cycle progression is tightly regulated to prevent uncontrolled growth and division. Specific cell cycle factors are responsible for driving the cell from one cell cycle stage to the next. Many of these proteins are targeted for degradation by the ubiquitin proteasome system when their function is no longer required or may disrupt cell cycle progression. Here we describe a series of experiments used to study the ubiquitinmediated degradation of cell cycle proteins. This collection of assays may be used to determine the requirement for individual proteins in the degradation and ubiquitination of cell cycle proteins in Saccharomyces cerevisiae.

Original languageEnglish (US)
Title of host publicationCell Cycle Control
Subtitle of host publicationMechanisms and Protocols
PublisherHumana Press Inc.
Pages343-356
Number of pages14
ISBN (Print)9781493908875
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1170
ISSN (Print)1064-3745

Keywords

  • Cell cycle
  • Degradation
  • Proteasome
  • Protein stability assay
  • Proteolysis
  • Ubiquitin

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