TY - JOUR
T1 - Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos
AU - Thyagarajan, Bharat
AU - Howard, Annie Green
AU - Durazo-Arvizu, Ramon
AU - Eckfeldt, John H.
AU - Gellman, Marc D.
AU - Kim, Ryung S.
AU - Liu, Kiang
AU - Mendez, Armando J.
AU - Penedo, Frank J.
AU - Talavera, Gregory A.
AU - Youngblood, Marston E.
AU - Zhao, Lihui
AU - Sotres-Alvarez, Daniela
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.
AB - Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.
KW - Analytical variation
KW - Biomarker variability
KW - Hispanics
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U2 - 10.1016/j.cca.2016.10.019
DO - 10.1016/j.cca.2016.10.019
M3 - Article
C2 - 27756543
AN - SCOPUS:84994013944
SN - 0009-8981
VL - 463
SP - 129
EP - 137
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -