Analytical advantages of highly stable stationary phases for reversed-phase LC

Clayton McNeff, Lorinda Zigan, Kerry Johnson, Peter W. Carr, Aosheng Wang, Anne M. Weber-Main

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


During the past five years, many manufacturers of high performance liquid chromatography (HPLC) columns have focused on improving stationary-phase stability and reproducibility. Improved column stability - both chemical and thermal - offers new advantages, such as decreased analysis time and new methods of selectivity optimization. More stable HPLC packing materials have been achieved through advances in silane chemistry; however, the greatest improvements in stability have resulted from the use of alternative nonsilica supports such as synthetic organic polymers, alumina, and zirconia. In this article, the authors describe their use of various test solutes to compare the efficiency, selectivity, and hydrophobic retention mechanisms of five commercially available HPLC columns based on silica, alumina, zirconia, and polystyrene cross-linked with divinylbenzene (PS-DVB).

Original languageEnglish (US)
Pages (from-to)514-529
Number of pages16
JournalLCGC North America
Issue number5
StatePublished - May 2000


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