Analysis of Viruses in the Cellular Context by Electron Tomography

Peijun Zhang, Luiza Mendonça

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Electron microscopy is a powerful tool to study viral infection in the cellular context. A vast majority of our ultrastructural understanding of cellular infection has been acquired through imaging of chemically fixed, plastic embedded samples using traditional electron microscopy. Application of electron tomography to such specimens enables ultrastructural analysis of virus infection in three-dimensions. Advances in cryo-fixation and cryo-electron microscopy and tomography have allowed investigation of viruses in the cellular context under near-native frozen-hydrated conditions, pushing structural analysis to higher resolutions, especially when combined with sub-tomogram averaging of multiple copies of the repeating unit.

Original languageEnglish (US)
Title of host publicationEncyclopedia of Virology
Subtitle of host publicationVolume 1-5, Fourth Edition
Number of pages6
ISBN (Electronic)9780128145166
StatePublished - Jan 1 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Ltd. All rights reserved


  • 3DEM
  • Cell
  • Cryo
  • Electron microscopy
  • Electron tomography
  • High pressure freezing
  • Imaging
  • Infection
  • Viruses


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