Our purpose is to generalize the chief conclusion of earlier studies based on complex maize seed extract samples that indicated that at very short times (less than 5. min) the peak capacity of 1D liquid chromatography was better than that of comprehensive, on-line 2D liquid chromatography (LC. ×. LC), but that the LC. ×. LC method begins to out-perform the 1D method at surprisingly short analysis times of only 5-10. min. We call the analysis time at which the peak capacities of 1D and LC. ×. LC become equal the "crossover time." We quantify the importance of various system parameters such as the 1D peak capacity, the second dimension cycle time and second dimension peak capacity, and fractional coverage of the two-dimensional separation space on the crossover time of 1D versus LC. ×. LC chromatography. For ranges of these parameters used in our earlier experimental studies, we believe that crossover times will generally be between 3 and 8. min under optimized on-line LC. ×. LC conditions.
Bibliographical noteFunding Information:
This work was supported by grants from the National Institutes of Health (Grant 5R01GM54585-15), and from the National Science Foundation (Grant CHE-0911330).
- Analysis time
- Crossover time
- Cycle time
- Fractional coverage
- Peak capacity