Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography

Charles A. Lucy; Peter W. Carr

doi:10.1016/S0021-9673(01)96218-1

Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography

Charles A. Lucy, Peter W. Carr

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.

Original language	English (US)
Pages (from-to)	159-168
Number of pages	10
Journal	Journal of Chromatography A
Volume	556
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(01)96218-1
State	Published - Sep 6 1991

Bibliographical note

Funding Information:
This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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10.1016/S0021-9673(01)96218-1

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title = "Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography",

abstract = "In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.",

author = "Lucy, {Charles A.} and Carr, {Peter W.}",

note = "Funding Information: This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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AU - Lucy, Charles A.

AU - Carr, Peter W.

N1 - Funding Information: This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/9/6

Y1 - 1991/9/6

N2 - In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.

AB - In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.

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ER -

Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography

Abstract

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