In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.
Bibliographical noteFunding Information:
This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota.
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