TY - JOUR
T1 - Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography
AU - Lucy, Charles A.
AU - Carr, Peter W.
N1 - Funding Information:
This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota.
PY - 1991/9/6
Y1 - 1991/9/6
N2 - In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.
AB - In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.
UR - http://www.scopus.com/inward/record.url?scp=0025774115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025774115&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)96218-1
DO - 10.1016/S0021-9673(01)96218-1
M3 - Article
AN - SCOPUS:0025774115
SN - 0021-9673
VL - 556
SP - 159
EP - 168
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -