Analysis of the separability of plate height into overload and intrinsic contributions using the kinetic model of non-linear chromatography

Charles A. Lucy, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In developing optimization strategies for preparative-scale chromatography it is very convenient, if not entirely valid, to the represent overall peak broadening in terms of the sum of two distinct, independent contributions to the plate height: that portion due to band broadening under linear chromatographic conditions and that due to the effect of mass overload. The kinetic model of non-linear elution chromatography is used to demonstrate that this separation of terms is a reasonable approximation under a wide range of chromatographic conditions and to define the limits of this approximation.

Original languageEnglish (US)
Pages (from-to)159-168
Number of pages10
JournalJournal of Chromatography A
Volume556
Issue number1-2
DOIs
StatePublished - Sep 6 1991

Bibliographical note

Funding Information:
This work was supported in part by the Natural Sciences and Engineering Research Council of Canada and by the NSF Center for Bioprocess Technology. University of Minnesota.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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