Analiz chetvertichnoǐ struktury sekretiruemoǐ repressibel'noǐ kisloǐ fosfatazy drozhzheǐ Saccharomyces cerevisiae.

Translated title of the contribution: Analysis of the quaternary structure of secreted repressible acid phosphatase from the yeast Saccharomyces cerevisiae

M. G. Shnyreva, V. L. Tsuprun, V. I. Stel'mashchuk, S. N. Egorov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The structural organization of extracellular repressible acid phosphatase from S. cerevisiae has been studied. The existence of multiple acid phosphatase forms with isoelectric points at pH 4.1-4.8 has been confirmed by isoelectrofocusing. The molecular masses of three acid phosphatase isoforms (56, 57-59, and 60 kDa) obtained after enzymatic deglycosylation correlate with the data obtained previously during the analysis of translation products in cell-free systems. Electron microscopic studies revealed that the acid phosphatase molecule has a square shape and is made up of four identical subunits with molecular masses of about 125 kDa.

Translated title of the contributionAnalysis of the quaternary structure of secreted repressible acid phosphatase from the yeast Saccharomyces cerevisiae
Original languageRussian
Pages (from-to)1100-1108
Number of pages9
JournalBiokhimiya
Volume57
Issue number7
StatePublished - Jul 1 1992

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