Analysis of the nucleotide sequence of the guinea pig cytomegalovirus (GPCMV) genome

Mark R Schleiss, Alistair McGregor, K. Yeon Choi, Shailesh V. Date, Xiaohong Cui, Michael A. McVoy

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Abstract

In this report we describe the genomic sequence of guinea pig cytomegalovirus (GPCMV) assembled from a tissue culture-derived bacterial artificial chromosome clone, plasmid clones of viral restriction fragments, and direct PCR sequencing of viral DNA. The GPCMV genome is 232,678 bp, excluding the terminal repeats, and has a GC content of 55%. A total of 105 open reading frames (ORFs) of > 100 amino acids with sequence and/or positional homology to other CMV ORFs were annotated. Positional and sequence homologs of human cytomegalovirus open reading frames UL23 through UL122 were identified. Homology with other cytomegaloviruses was most prominent in the central ∼60% of the genome, with divergence of sequence and lack of conserved homologs at the respective genomic termini. Of interest, the GPCMV genome was found in many cases to bear stronger phylogenetic similarity to primate CMVs than to rodent CMVs. The sequence of GPCMV should facilitate vaccine and pathogenesis studies in this model of congenital CMV infection.

Original languageEnglish (US)
Article number139
JournalVirology journal
Volume5
DOIs
StatePublished - 2008

Bibliographical note

Funding Information:
Grant support was provided from NIH HD044864-01 and HD38416-01 (to MRS) and R01AI46668 (to MAM). The authors acknowledge helpful discussions and input from Becket Feierbach (Genentech, Inc.). The authors also acknowledge the technical contributions of Yonggen Song and the gift of the Hind III "D" plasmid from HC Isom, Penn State University.

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