Analysis of the effects of fatty acids and related compounds on the synthesis of phosphatidylcholine in lymphocytes

Donna S. Whitlon, Kristin E. Anderson, Gerald C. Mueller

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Phosphatidylcholine synthesis in cultured bovine lymphocytes is stimulated by cis-unsaturated fatty acids. This stimulation is correlated with an activation of the enzyme cytidyltransferase (EC 2.7.7.15) and its apparent translocation from the cytosol to the membrane/particulate of cells. In addition, these agents increase the levels of cytidine diphosphocholine - a product of the cytidyltransferase reaction and a precursor to phosphatidylcholine. Retinoic acid and 5,8,11,14-eicosatetraynoic acid both activate cytidyltransferase activity and raise cytidine diphosphocholine levels, yet they are ineffective as stimulators of overall phosphatidylcholine synthesis. The effects of all of these lipids are reversed by the delayed addition of bovine serum albumin. The data point to the view that cytidyltransferase activation is required but is not sufficient for stimulation of phosphatidylcholine synthesis: regulation at another step is suggested.

Original languageEnglish (US)
Pages (from-to)369-377
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume835
Issue number2
DOIs
StatePublished - Jul 9 1985

Bibliographical note

Funding Information:
for their technical assistance,a nd Mary LeMahieu for help in the preparation of the manuscript. This work was supported by Grants P30-CA-07175 and POl-CA-23076 from the National Cancer Institute, USPHS, and NIH Training Grant T32-CA-09135. G.C.M. is the recipient of a research career award from the National Cancer Institute.

Keywords

  • (Bovine lymphocyte)
  • Cytidyltransferase
  • Fatty acid
  • Phosphatidylcholine
  • Phospholipid synthesis

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