TY - JOUR
T1 - Analysis of syn- and anti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene-deoxyribonucleoside adducts by boronate chromatography
AU - Melikian, Assieh A.
AU - Hecht, Stephen S.
AU - Hoffmann, Dietrich
AU - Pataki, John
AU - Harvey, Ronald G.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The Servacel DHB (m-dihydroxyborylphenylaminoethyl cellulose) chromatographic procedure developed by Sawiki et al. (Cancer Res., 43, 3212-3218, 1983) for analysis of 7,12-dimethylbenz[a]anthracene (DMBA)-DNA adducts was applied to analyze syn- and anti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene (DE-I)-deoxyribonucleoside adducts. Identical elution conditions to whose developed for the DMBA adducts were employed. While the results were similar to those obtained in the DMBA system, some of the anti-DE-I-deoxyribonucleoside adducts eluted with the buffer system used for elution of syn-adducts. Complete resolution of the anti- and syn-adducts was obtained when modified elution conditions as developed by Pruess-Schwartz et al. (Cancer Res., 44, 4104-4110, 1984) for analysis of syn- and anti-7α-8β-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts were applied. Based on this chromatographic procedure about 15% of the DE-I deoxyribonucleoside adducts, formed in mouse skin DNA upon treatment with 5-[3H]methylchrysene (MeC), originated from syn-DE-I.
AB - The Servacel DHB (m-dihydroxyborylphenylaminoethyl cellulose) chromatographic procedure developed by Sawiki et al. (Cancer Res., 43, 3212-3218, 1983) for analysis of 7,12-dimethylbenz[a]anthracene (DMBA)-DNA adducts was applied to analyze syn- and anti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene (DE-I)-deoxyribonucleoside adducts. Identical elution conditions to whose developed for the DMBA adducts were employed. While the results were similar to those obtained in the DMBA system, some of the anti-DE-I-deoxyribonucleoside adducts eluted with the buffer system used for elution of syn-adducts. Complete resolution of the anti- and syn-adducts was obtained when modified elution conditions as developed by Pruess-Schwartz et al. (Cancer Res., 44, 4104-4110, 1984) for analysis of syn- and anti-7α-8β-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts were applied. Based on this chromatographic procedure about 15% of the DE-I deoxyribonucleoside adducts, formed in mouse skin DNA upon treatment with 5-[3H]methylchrysene (MeC), originated from syn-DE-I.
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U2 - 10.1016/0304-3835(85)90012-6
DO - 10.1016/0304-3835(85)90012-6
M3 - Article
SN - 0304-3835
VL - 27
SP - 91
EP - 97
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -
