Analysis of selenium in bovine liver by a gas chromatography with mass-selective, electron-capture and nitrogen-phosphorus detection

Ashok K Singh, Thomas White, Tom Arendt, Yin Jiang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The concentration of selenium (Se) in liver was determined by gas chromatography (GC) with mass-selective (GC-MS), electron capture (GC-ECD) and nitrogen-phosphorus (GC-NPD) detection. Liver samples were digested in a mixture containing HNO3 and Mg(NO3). Se(VI) was converted to Se(IV). Se(IV) was derivatized with 4-nitrophenylenediamine and then extracted in toluene. A 1-μl volume of the toluene extract was analyzed by the GC-MS, GC-ECD or GC-NPD methods. The detection limits of the GC-ECD, the GC-NPD and the GC-MS methods were 25, 50 and 800 pg, respectively. The GC-NPD method was more selective for the derivatized Se than the GC-ECD method. The GC-MS method had the advantage of using the 70Se isotope as the internal standard. Se concentrations in liver samples determined by the three methods were comparable.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume690
Issue number1-2
DOIs
StatePublished - Mar 7 1997

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Selenium
Gas chromatography
Liver
Phosphorus
Nitrogen
Electrons
Toluene
Isotopes

Keywords

  • Selenium

Cite this

Analysis of selenium in bovine liver by a gas chromatography with mass-selective, electron-capture and nitrogen-phosphorus detection. / Singh, Ashok K; White, Thomas; Arendt, Tom; Jiang, Yin.

In: Journal of Chromatography B: Biomedical Applications, Vol. 690, No. 1-2, 07.03.1997, p. 327-331.

Research output: Contribution to journalArticle

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