Analysis of miRNA expression profiling in melatonin-exposured endothelial cells

Gun Woo Son, Hana Yang, Hye Rim Park, Seung Eun Lee, Young Ho Jin, Cheung Seog Park, Yong Seek Park

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Melatonin is produced from tryptophan and is secreted by the pineal gland in periods of darkness; it has cytoprotective effects in endothelial cells. It has been reported that melatonin mediates the regulation of gene expression at the post-transcriptional level through its effect on miRNA expression. miRNAs are small non-coding RNAs that silence gene expression, regulate most cellular processes involved in development, and maintain cell functions. In this study, we confirmed the expression of 136 and 154 miRNAs when endothelial cells were treated with melatonin concentrations of 100 μM and 1 mM, respectively. We investigated anti-correlations in miRNA-target-mRNA pairs, and we identified a total of 13 and 44 mRNAs showing an expression difference of >1.5 fold, following exposure to melatonin concentrations of 100 μM and 1 mM, respectively. These profiling data revealed a variety of biological effects, including cell death, apoptosis and proliferation, via a Gene Ontology analysis of biological processes. The results of this study suggest that melatonin may regulate miRNA and gene expression and may have a cytoprotective effect on endothelial cells.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalMolecular and Cellular Toxicology
Volume12
Issue number1
DOIs
StatePublished - Mar 1 2016

Keywords

  • Endothelial cells
  • Melatonin
  • miRNA expression profiling

Fingerprint Dive into the research topics of 'Analysis of miRNA expression profiling in melatonin-exposured endothelial cells'. Together they form a unique fingerprint.

Cite this