Analysis of K99 plasmids from enterotoxigenic Escherichia coli

Richard E. Isaacson; Gail L. Start

doi:10.1016/0378-1097(92)90618-X

Analysis of K99 plasmids from enterotoxigenic Escherichia coli

Richard E. Isaacson, Gail L. Start

Microbiome and Antibiotic Resistance

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P^- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.

Original language	English (US)
Pages (from-to)	141-146
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	90
Issue number	2
DOIs	https://doi.org/10.1016/0378-1097(92)90618-X
State	Published - Jan 1 1992

Bibliographical note

Funding Information:
This matcrial is based upon work supported by the Cooperative State Research Service, U.S. Department of Agriculture under agreement No. 9001678.

Keywords

Adhesion
Enterotoxigenic
Epidemiology
Escherichia coli
Pili
Restriction fragments
Southern hybridization

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10.1016/0378-1097(92)90618-X

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title = "Analysis of K99 plasmids from enterotoxigenic Escherichia coli",

abstract = "Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.",

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author = "Isaacson, {Richard E.} and Start, {Gail L.}",

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T1 - Analysis of K99 plasmids from enterotoxigenic Escherichia coli

AU - Isaacson, Richard E.

AU - Start, Gail L.

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PY - 1992/1/1

Y1 - 1992/1/1

N2 - Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.

AB - Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.

KW - Adhesion

KW - Enterotoxigenic

KW - Epidemiology

KW - Escherichia coli

KW - Pili

KW - Restriction fragments

KW - Southern hybridization

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JF - FEMS Microbiology Letters

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ER -

Analysis of K99 plasmids from enterotoxigenic Escherichia coli

Abstract

Bibliographical note

Keywords

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