Analysis of K99 plasmids from enterotoxigenic Escherichia coli

Richard E. Isaacson, Gail L. Start

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalFEMS Microbiology Letters
Volume90
Issue number2
DOIs
StatePublished - Jan 1 1992

Bibliographical note

Funding Information:
This matcrial is based upon work supported by the Cooperative State Research Service, U.S. Department of Agriculture under agreement No. 9001678.

Keywords

  • Adhesion
  • Enterotoxigenic
  • Epidemiology
  • Escherichia coli
  • Pili
  • Restriction fragments
  • Southern hybridization

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