Analysis of immunoglobulin and T-cell receptor gene rearrangements in human fetal bone marrow B lineage cells

Tucker W LeBien, R. L. Elstrom, M. Moseley, J. H. Kersey, F. Griesinger

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Fetal bone marrow B lineage cells representing multiple stages of B cell development were isolated by two-color cell sorting and analyzed for immunoglobulin H and T-cell receptor (TCR) gamma and delta gene rearrangements. Analysis of CD10+/surface mu- cells using a J(H) probe revealed a high frequency of rearrangements; some of these rearrangements used the 3' D region gene DQ52. Analysis of CD10+/surface mu- cells revealed no detectable TCR-γ or -δ rearrangements, nor were TCR-δ rearrangements detected in CD10+/surface mu+ cells, despite the limited repertoire of these genes. These observations are surprising given the high frequency of TCR δ/γ rearrangements in B cell precursor acute lymphoblastic leukemia, and identify a potential difference in patterns of gene rearrangement that distinguish normal and leukemic B cell precursors.

Original languageEnglish (US)
Pages (from-to)1196-1200
Number of pages5
JournalBlood
Volume76
Issue number6
DOIs
StatePublished - 1990

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