Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3

Tucker Lebien; John Kersey; Shinpei Nakazawa; Keisuke Minato; Jun Minowada

doi:10.1016/0145-2126(82)90091-1

Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3

Tucker Lebien
, John Kersey
, Shinpei Nakazawa
, Keisuke Minato
, Jun Minowada

Health Sciences Administration

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

A panel of monoclonal antibodies (BA-1, BA-2 and BA-3) were made against the pre-B acute lymphoblastic leukemia (ALL) cell line NALM-6. BA-3 binds to the 100,000 mol. wt common ALL antigen (gp100/CALLA), BA-2 binds to a 24,000 mol. wt (p24) cell surface structure and BA-1 binds to a currently undefined antigen. These antibodies were analysed for their reactivity with 67 human leukemia/lymphoma cell lines. The cell lines studied included T ALL, non-T ALL. surface immunoglobulin⁺ leukemias/lymphomas, and myeloid/monocytoid leukemias. The results indicate that all three antibodies react primarily (but not exclusively) with malignant cells early in B cell development. This pattern of reactivity was similar to previous results obtained with fresh, non-cultured malignant cells. BA-1, BA-2 and BA-3 are useful tools for analysing the developmental options of normal lymphoid cells and for "cleaning up" leukemia/lymphoma cells in selected cases of autologous bone marrow transplantation.

Original language	English (US)
Pages (from-to)	299-305
Number of pages	7
Journal	Leukemia research
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/0145-2126(82)90091-1
State	Published - 1982

Bibliographical note

Funding Information:
Supported by grants CA-25097, CA-14413. contract CB-84261 and New Investigator Award CA-28526 (to T.W.LI from the National Cancer Institute, National Institutes of Health, and by a grant from the University of Minnesota Graduate School. Ahhret'iations: ALL. acute lymphoblastic leukemia; Ig, immunoglobulin; TdT, terminal deoxynucleotidyl transferase: CALLA. common ALL antigen; NHL, non-Hodgkin's lymphoma; BL, Burkitt's lymphoma. Correspondence to: Dr. T. LeBien, Box 609 Mayo, Department of Laboratory Medicine and Pathology° University of Minnesota, Minneapolis, MN 55455, U.S.A.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Monoclonal antibodies
leukemia/lymphoma cell lines

Publisher link

10.1016/0145-2126(82)90091-1

Find It

Find It at U of M Libraries

Cite this

@article{ebc6afdc9bf04a72abc1d627f5159f06,

title = "Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3",

abstract = "A panel of monoclonal antibodies (BA-1, BA-2 and BA-3) were made against the pre-B acute lymphoblastic leukemia (ALL) cell line NALM-6. BA-3 binds to the 100,000 mol. wt common ALL antigen (gp100/CALLA), BA-2 binds to a 24,000 mol. wt (p24) cell surface structure and BA-1 binds to a currently undefined antigen. These antibodies were analysed for their reactivity with 67 human leukemia/lymphoma cell lines. The cell lines studied included T ALL, non-T ALL. surface immunoglobulin+ leukemias/lymphomas, and myeloid/monocytoid leukemias. The results indicate that all three antibodies react primarily (but not exclusively) with malignant cells early in B cell development. This pattern of reactivity was similar to previous results obtained with fresh, non-cultured malignant cells. BA-1, BA-2 and BA-3 are useful tools for analysing the developmental options of normal lymphoid cells and for {"}cleaning up{"} leukemia/lymphoma cells in selected cases of autologous bone marrow transplantation.",

keywords = "Monoclonal antibodies, leukemia/lymphoma cell lines",

author = "Tucker Lebien and John Kersey and Shinpei Nakazawa and Keisuke Minato and Jun Minowada",

note = "Funding Information: Supported by grants CA-25097, CA-14413. contract CB-84261 and New Investigator Award CA-28526 (to T.W.LI from the National Cancer Institute, National Institutes of Health, and by a grant from the University of Minnesota Graduate School. Ahhret'iations: ALL. acute lymphoblastic leukemia; Ig, immunoglobulin; TdT, terminal deoxynucleotidyl transferase: CALLA. common ALL antigen; NHL, non-Hodgkin's lymphoma; BL, Burkitt's lymphoma. Correspondence to: Dr. T. LeBien, Box 609 Mayo, Department of Laboratory Medicine and Pathology° University of Minnesota, Minneapolis, MN 55455, U.S.A.",

year = "1982",

doi = "10.1016/0145-2126(82)90091-1",

language = "English (US)",

volume = "6",

pages = "299--305",

journal = "Leukemia research",

issn = "0145-2126",

publisher = "Elsevier Ltd",

number = "3",

}

TY - JOUR

T1 - Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3

AU - Lebien, Tucker

AU - Kersey, John

AU - Nakazawa, Shinpei

AU - Minato, Keisuke

AU - Minowada, Jun

N1 - Funding Information: Supported by grants CA-25097, CA-14413. contract CB-84261 and New Investigator Award CA-28526 (to T.W.LI from the National Cancer Institute, National Institutes of Health, and by a grant from the University of Minnesota Graduate School. Ahhret'iations: ALL. acute lymphoblastic leukemia; Ig, immunoglobulin; TdT, terminal deoxynucleotidyl transferase: CALLA. common ALL antigen; NHL, non-Hodgkin's lymphoma; BL, Burkitt's lymphoma. Correspondence to: Dr. T. LeBien, Box 609 Mayo, Department of Laboratory Medicine and Pathology° University of Minnesota, Minneapolis, MN 55455, U.S.A.

PY - 1982

Y1 - 1982

N2 - A panel of monoclonal antibodies (BA-1, BA-2 and BA-3) were made against the pre-B acute lymphoblastic leukemia (ALL) cell line NALM-6. BA-3 binds to the 100,000 mol. wt common ALL antigen (gp100/CALLA), BA-2 binds to a 24,000 mol. wt (p24) cell surface structure and BA-1 binds to a currently undefined antigen. These antibodies were analysed for their reactivity with 67 human leukemia/lymphoma cell lines. The cell lines studied included T ALL, non-T ALL. surface immunoglobulin+ leukemias/lymphomas, and myeloid/monocytoid leukemias. The results indicate that all three antibodies react primarily (but not exclusively) with malignant cells early in B cell development. This pattern of reactivity was similar to previous results obtained with fresh, non-cultured malignant cells. BA-1, BA-2 and BA-3 are useful tools for analysing the developmental options of normal lymphoid cells and for "cleaning up" leukemia/lymphoma cells in selected cases of autologous bone marrow transplantation.

AB - A panel of monoclonal antibodies (BA-1, BA-2 and BA-3) were made against the pre-B acute lymphoblastic leukemia (ALL) cell line NALM-6. BA-3 binds to the 100,000 mol. wt common ALL antigen (gp100/CALLA), BA-2 binds to a 24,000 mol. wt (p24) cell surface structure and BA-1 binds to a currently undefined antigen. These antibodies were analysed for their reactivity with 67 human leukemia/lymphoma cell lines. The cell lines studied included T ALL, non-T ALL. surface immunoglobulin+ leukemias/lymphomas, and myeloid/monocytoid leukemias. The results indicate that all three antibodies react primarily (but not exclusively) with malignant cells early in B cell development. This pattern of reactivity was similar to previous results obtained with fresh, non-cultured malignant cells. BA-1, BA-2 and BA-3 are useful tools for analysing the developmental options of normal lymphoid cells and for "cleaning up" leukemia/lymphoma cells in selected cases of autologous bone marrow transplantation.

KW - Monoclonal antibodies

KW - leukemia/lymphoma cell lines

UR - https://www.scopus.com/pages/publications/0019987517

UR - https://www.scopus.com/pages/publications/0019987517#tab=citedBy

U2 - 10.1016/0145-2126(82)90091-1

DO - 10.1016/0145-2126(82)90091-1

M3 - Article

C2 - 6956782

AN - SCOPUS:0019987517

SN - 0145-2126

VL - 6

SP - 299

EP - 305

JO - Leukemia research

JF - Leukemia research

IS - 3

ER -

Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3

Abstract

Bibliographical note

UN SDGs

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this