Analysis of histones and histone variants in plants

Ila Trivedi; Krishan Mohan Rai; Sunil Kumar Singh; Verandra Kumar; Mala Singh; Amol Ranjan; Niraj Lodhi; Samir V Sawant

doi:10.1007/978-1-61779-477-3_14

Analysis of histones and histone variants in plants

Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh, Verandra Kumar, Mala Singh, Amol Ranjan, Niraj Lodhi, Samir V Sawant

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.

Original language	English (US)
Pages (from-to)	225-36
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	833
DOIs	https://doi.org/10.1007/978-1-61779-477-3_14
State	Published - 2012

Keywords

Acids
Blotting, Western
Chemical Fractionation
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Electrophoresis, Polyacrylamide Gel
Histones/isolation & purification
Molecular Biology/methods
Molecular Weight
Mutant Proteins/isolation & purification
Plant Proteins/isolation & purification
Plants/metabolism
Silver Staining

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1007/978-1-61779-477-3_14

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title = "Analysis of histones and histone variants in plants",

abstract = "Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.",

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author = "Ila Trivedi and Rai, {Krishan Mohan} and Singh, {Sunil Kumar} and Verandra Kumar and Mala Singh and Amol Ranjan and Niraj Lodhi and Sawant, {Samir V}",

year = "2012",

doi = "10.1007/978-1-61779-477-3_14",

language = "English (US)",

volume = "833",

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journal = "Methods in molecular biology (Clifton, N.J.)",

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TY - JOUR

T1 - Analysis of histones and histone variants in plants

AU - Trivedi, Ila

AU - Rai, Krishan Mohan

AU - Singh, Sunil Kumar

AU - Kumar, Verandra

AU - Singh, Mala

AU - Ranjan, Amol

AU - Lodhi, Niraj

AU - Sawant, Samir V

PY - 2012

Y1 - 2012

N2 - Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.

AB - Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.

KW - Acids

KW - Blotting, Western

KW - Chemical Fractionation

KW - Chromatography, High Pressure Liquid

KW - Chromatography, Reverse-Phase

KW - Electrophoresis, Polyacrylamide Gel

KW - Histones/isolation & purification

KW - Molecular Biology/methods

KW - Molecular Weight

KW - Mutant Proteins/isolation & purification

KW - Plant Proteins/isolation & purification

KW - Plants/metabolism

KW - Silver Staining

U2 - 10.1007/978-1-61779-477-3_14

DO - 10.1007/978-1-61779-477-3_14

M3 - Article

C2 - 22183597

SN - 1064-3745

VL - 833

SP - 225

EP - 236

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Analysis of histones and histone variants in plants

Abstract

Keywords

PubMed: MeSH publication types

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