Analysis of histones and histone variants in plants

Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh, Verandra Kumar, Mala Singh, Amol Ranjan, Niraj Lodhi, Samir V Sawant

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.

Original languageEnglish (US)
Pages (from-to)225-36
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume833
DOIs
StatePublished - 2012

Fingerprint

Histones
Chromatin
Genome
Proteins
Ubiquitination
Eukaryotic Cells
Acetylation
Methylation
Phosphorylation
Genes

Keywords

  • Acids
  • Blotting, Western
  • Chemical Fractionation
  • Chromatography, High Pressure Liquid
  • Chromatography, Reverse-Phase
  • Electrophoresis, Polyacrylamide Gel
  • Histones/isolation & purification
  • Molecular Biology/methods
  • Molecular Weight
  • Mutant Proteins/isolation & purification
  • Plant Proteins/isolation & purification
  • Plants/metabolism
  • Silver Staining

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Trivedi, I., Rai, K. M., Singh, S. K., Kumar, V., Singh, M., Ranjan, A., ... Sawant, S. V. (2012). Analysis of histones and histone variants in plants. Methods in molecular biology (Clifton, N.J.), 833, 225-36. https://doi.org/10.1007/978-1-61779-477-3_14

Analysis of histones and histone variants in plants. / Trivedi, Ila; Rai, Krishan Mohan; Singh, Sunil Kumar; Kumar, Verandra; Singh, Mala; Ranjan, Amol; Lodhi, Niraj; Sawant, Samir V.

In: Methods in molecular biology (Clifton, N.J.), Vol. 833, 2012, p. 225-36.

Research output: Contribution to journalArticle

Trivedi, I, Rai, KM, Singh, SK, Kumar, V, Singh, M, Ranjan, A, Lodhi, N & Sawant, SV 2012, 'Analysis of histones and histone variants in plants', Methods in molecular biology (Clifton, N.J.), vol. 833, pp. 225-36. https://doi.org/10.1007/978-1-61779-477-3_14
Trivedi, Ila ; Rai, Krishan Mohan ; Singh, Sunil Kumar ; Kumar, Verandra ; Singh, Mala ; Ranjan, Amol ; Lodhi, Niraj ; Sawant, Samir V. / Analysis of histones and histone variants in plants. In: Methods in molecular biology (Clifton, N.J.). 2012 ; Vol. 833. pp. 225-36.
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