Analysis of Glycosaminoglycans in Stem Cell Glycomics

Boyangzi Li; Haiying Liu; Zhenqing Zhang; Hope E. Stansfield; Jonathan S. Dordick; Robert J. Linhardt

doi:10.1007/978-1-60761-962-8_19

Analysis of Glycosaminoglycans in Stem Cell Glycomics

Boyangzi Li
, Haiying Liu
, Zhenqing Zhang
, Hope E. Stansfield
, Jonathan S. Dordick
, Robert J. Linhardt

Research output: Chapter in Book/Report/Conference proceeding › Chapter

12 Scopus citations

Abstract

Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

Original language	English (US)
Title of host publication	Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	285-300
Number of pages	16
ISBN (Print)	9781607619611
DOIs	https://doi.org/10.1007/978-1-60761-962-8_19
State	Published - 2011
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	690
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Keywords

Chondroitin sulfate
Dermatan sulfate
Disaccharide analysis
Enzymatic digestion
Glycosaminoglycans
Heparan sulfate
Heparin
LC-MS
NCCIT cells
Purification

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10.1007/978-1-60761-962-8_19

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abstract = "Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.",

keywords = "Chondroitin sulfate, Dermatan sulfate, Disaccharide analysis, Enzymatic digestion, Glycosaminoglycans, Heparan sulfate, Heparin, LC-MS, NCCIT cells, Purification",

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AU - Liu, Haiying

AU - Zhang, Zhenqing

AU - Stansfield, Hope E.

AU - Dordick, Jonathan S.

AU - Linhardt, Robert J.

PY - 2011

Y1 - 2011

N2 - Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

AB - Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

KW - Chondroitin sulfate

KW - Dermatan sulfate

KW - Disaccharide analysis

KW - Enzymatic digestion

KW - Glycosaminoglycans

KW - Heparan sulfate

KW - Heparin

KW - LC-MS

KW - NCCIT cells

KW - Purification

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UR - https://www.scopus.com/pages/publications/79952197167#tab=citedBy

U2 - 10.1007/978-1-60761-962-8_19

DO - 10.1007/978-1-60761-962-8_19

M3 - Chapter

C2 - 21043000

AN - SCOPUS:79952197167

SN - 9781607619611

T3 - Methods in Molecular Biology

SP - 285

EP - 300

BT - Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

PB - Humana Press Inc.

ER -

Analysis of Glycosaminoglycans in Stem Cell Glycomics

Abstract

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Bibliographical note

Keywords

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